Title : Quantitation of DNA methylation by melt curve analysis

In the submitted manuscript entitled “Quantitation of methylation by melt curve analysis”, the authors provided a novel method to quantitate DNA methylation using the raw fluorescence melt data obtainable following PCR amplification of bisulfite modified DNA. The development of a rapid, simple, low-cost, convenient and reliable method to quauntify methylation is certainly important. However, based on the accomplishments of the current version, the manuscript is not sufficiently satisfied to be accepted for publication in BMC cancer.